Abstract
Alzheimer's disease is a significant global health issue, and studies suggest that neuroinflammation plays a vital role in the advancement of this disease. In this study, anakinra has been shown to display a time- and concentration-dependent antineuroinflammatory effect. In the in vitro studies, it diminished the gene expressions of tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO) synthase 2 stimulated by lipopolysaccharide (LPS). Anakinra also reduced the LPS-induced production of NO and reactive oxygen species. Thus, the hypertrophic state of LPS-activated BV2 microglial cells was reversed by anakinra. Furthermore, acrylamide (ACR)-induced activation of nuclear transcription factor-κB, TNF-α, and interleukin-1β was downregulated, while cAMP response element binding protein and brain-derived neurotrophic factor expression levels were markedly enhanced in ACR-treated zebrafish larvae. It was also observed that anakinra improved the uncoordinated swimming behaviors in ACR-exposed zebrafish larvae. Overall, anakinra demonstrated potential antineuroinflammatory and antioxidative effects.
| Original language | English |
|---|---|
| Journal | ACS Chemical Neuroscience |
| DOIs | |
| Publication status | Published - 30 Aug 2024 |
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Retinasamy, T., Lee, A. L. Y., Lee, H. S., Lee, V. L. L., Shaikh, M. F., & Yeong, K. Y. (2024). Repurposing Anakinra for Alzheimer's Disease: The In Vitro and In Vivo Effects of Anakinra on LPS- and AC-Induced Neuroinflammation. ACS Chemical Neuroscience. https://doi.org/10.1021/acschemneuro.4c00205
Retinasamy, Thaarvena ; Lee, Amber Lot Yee ; Lee, Hsien Siang et al. / Repurposing Anakinra for Alzheimer's Disease: The In Vitro and In Vivo Effects of Anakinra on LPS- and AC-Induced Neuroinflammation. In: ACS Chemical Neuroscience. 2024.
@article{4f3c2356987c460da85e1b16cce3f07a,
title = "Repurposing Anakinra for Alzheimer's Disease: The In Vitro and In Vivo Effects of Anakinra on LPS- and AC-Induced Neuroinflammation.",
abstract = "Alzheimer's disease is a significant global health issue, and studies suggest that neuroinflammation plays a vital role in the advancement of this disease. In this study, anakinra has been shown to display a time- and concentration-dependent antineuroinflammatory effect. In the in vitro studies, it diminished the gene expressions of tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO) synthase 2 stimulated by lipopolysaccharide (LPS). Anakinra also reduced the LPS-induced production of NO and reactive oxygen species. Thus, the hypertrophic state of LPS-activated BV2 microglial cells was reversed by anakinra. Furthermore, acrylamide (ACR)-induced activation of nuclear transcription factor-κB, TNF-α, and interleukin-1β was downregulated, while cAMP response element binding protein and brain-derived neurotrophic factor expression levels were markedly enhanced in ACR-treated zebrafish larvae. It was also observed that anakinra improved the uncoordinated swimming behaviors in ACR-exposed zebrafish larvae. Overall, anakinra demonstrated potential antineuroinflammatory and antioxidative effects. ",
author = "Thaarvena Retinasamy and Lee, {Amber Lot Yee} and Lee, {Hsien Siang} and Lee, {Vanessa Lin Lin} and Shaikh, {Mohd Farooq} and Yeong, {Keng Yoon}",
year = "2024",
month = aug,
day = "30",
doi = "10.1021/acschemneuro.4c00205",
language = "English",
journal = "ACS Chemical Neuroscience",
issn = "1948-7193",
publisher = "American Chemical Society",
}
Retinasamy, T, Lee, ALY, Lee, HS, Lee, VLL, Shaikh, MF & Yeong, KY 2024, 'Repurposing Anakinra for Alzheimer's Disease: The In Vitro and In Vivo Effects of Anakinra on LPS- and AC-Induced Neuroinflammation.', ACS Chemical Neuroscience. https://doi.org/10.1021/acschemneuro.4c00205
Repurposing Anakinra for Alzheimer's Disease: The In Vitro and In Vivo Effects of Anakinra on LPS- and AC-Induced Neuroinflammation. / Retinasamy, Thaarvena; Lee, Amber Lot Yee; Lee, Hsien Siang et al.
In: ACS Chemical Neuroscience, 30.08.2024.
Research output: Contribution to journal › Article › peer-review
TY - JOUR
T1 - Repurposing Anakinra for Alzheimer's Disease: The In Vitro and In Vivo Effects of Anakinra on LPS- and AC-Induced Neuroinflammation.
AU - Retinasamy, Thaarvena
AU - Lee, Amber Lot Yee
AU - Lee, Hsien Siang
AU - Lee, Vanessa Lin Lin
AU - Shaikh, Mohd Farooq
AU - Yeong, Keng Yoon
PY - 2024/8/30
Y1 - 2024/8/30
N2 - Alzheimer's disease is a significant global health issue, and studies suggest that neuroinflammation plays a vital role in the advancement of this disease. In this study, anakinra has been shown to display a time- and concentration-dependent antineuroinflammatory effect. In the in vitro studies, it diminished the gene expressions of tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO) synthase 2 stimulated by lipopolysaccharide (LPS). Anakinra also reduced the LPS-induced production of NO and reactive oxygen species. Thus, the hypertrophic state of LPS-activated BV2 microglial cells was reversed by anakinra. Furthermore, acrylamide (ACR)-induced activation of nuclear transcription factor-κB, TNF-α, and interleukin-1β was downregulated, while cAMP response element binding protein and brain-derived neurotrophic factor expression levels were markedly enhanced in ACR-treated zebrafish larvae. It was also observed that anakinra improved the uncoordinated swimming behaviors in ACR-exposed zebrafish larvae. Overall, anakinra demonstrated potential antineuroinflammatory and antioxidative effects.
AB - Alzheimer's disease is a significant global health issue, and studies suggest that neuroinflammation plays a vital role in the advancement of this disease. In this study, anakinra has been shown to display a time- and concentration-dependent antineuroinflammatory effect. In the in vitro studies, it diminished the gene expressions of tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO) synthase 2 stimulated by lipopolysaccharide (LPS). Anakinra also reduced the LPS-induced production of NO and reactive oxygen species. Thus, the hypertrophic state of LPS-activated BV2 microglial cells was reversed by anakinra. Furthermore, acrylamide (ACR)-induced activation of nuclear transcription factor-κB, TNF-α, and interleukin-1β was downregulated, while cAMP response element binding protein and brain-derived neurotrophic factor expression levels were markedly enhanced in ACR-treated zebrafish larvae. It was also observed that anakinra improved the uncoordinated swimming behaviors in ACR-exposed zebrafish larvae. Overall, anakinra demonstrated potential antineuroinflammatory and antioxidative effects.
U2 - 10.1021/acschemneuro.4c00205
DO - 10.1021/acschemneuro.4c00205
M3 - Article
C2 - 39213521
SN - 1948-7193
JO - ACS Chemical Neuroscience
JF - ACS Chemical Neuroscience
ER -
Retinasamy T, Lee ALY, Lee HS, Lee VLL, Shaikh MF, Yeong KY. Repurposing Anakinra for Alzheimer's Disease: The In Vitro and In Vivo Effects of Anakinra on LPS- and AC-Induced Neuroinflammation. ACS Chemical Neuroscience. 2024 Aug 30. doi: 10.1021/acschemneuro.4c00205
