Repurposing Anakinra for Alzheimer's Disease: The In Vitro and In Vivo Effects of Anakinra on LPS- and AC-Induced Neuroinflammation. (2024)

Retinasamy, T., Lee, A. L. Y., Lee, H. S., Lee, V. L. L., Shaikh, M. F., & Yeong, K. Y. (2024). Repurposing Anakinra for Alzheimer's Disease: The In Vitro and In Vivo Effects of Anakinra on LPS- and AC-Induced Neuroinflammation. ACS Chemical Neuroscience. https://doi.org/10.1021/acschemneuro.4c00205

Retinasamy, Thaarvena ; Lee, Amber Lot Yee ; Lee, Hsien Siang et al. / Repurposing Anakinra for Alzheimer's Disease: The In Vitro and In Vivo Effects of Anakinra on LPS- and AC-Induced Neuroinflammation. In: ACS Chemical Neuroscience. 2024.

@article{4f3c2356987c460da85e1b16cce3f07a,

title = "Repurposing Anakinra for Alzheimer's Disease: The In Vitro and In Vivo Effects of Anakinra on LPS- and AC-Induced Neuroinflammation.",

abstract = "Alzheimer's disease is a significant global health issue, and studies suggest that neuroinflammation plays a vital role in the advancement of this disease. In this study, anakinra has been shown to display a time- and concentration-dependent antineuroinflammatory effect. In the in vitro studies, it diminished the gene expressions of tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO) synthase 2 stimulated by lipopolysaccharide (LPS). Anakinra also reduced the LPS-induced production of NO and reactive oxygen species. Thus, the hypertrophic state of LPS-activated BV2 microglial cells was reversed by anakinra. Furthermore, acrylamide (ACR)-induced activation of nuclear transcription factor-κB, TNF-α, and interleukin-1β was downregulated, while cAMP response element binding protein and brain-derived neurotrophic factor expression levels were markedly enhanced in ACR-treated zebrafish larvae. It was also observed that anakinra improved the uncoordinated swimming behaviors in ACR-exposed zebrafish larvae. Overall, anakinra demonstrated potential antineuroinflammatory and antioxidative effects. ",

author = "Thaarvena Retinasamy and Lee, {Amber Lot Yee} and Lee, {Hsien Siang} and Lee, {Vanessa Lin Lin} and Shaikh, {Mohd Farooq} and Yeong, {Keng Yoon}",

year = "2024",

month = aug,

day = "30",

doi = "10.1021/acschemneuro.4c00205",

language = "English",

journal = "ACS Chemical Neuroscience",

issn = "1948-7193",

publisher = "American Chemical Society",

}

Retinasamy, T, Lee, ALY, Lee, HS, Lee, VLL, Shaikh, MF & Yeong, KY 2024, 'Repurposing Anakinra for Alzheimer's Disease: The In Vitro and In Vivo Effects of Anakinra on LPS- and AC-Induced Neuroinflammation.', ACS Chemical Neuroscience. https://doi.org/10.1021/acschemneuro.4c00205

Repurposing Anakinra for Alzheimer's Disease: The In Vitro and In Vivo Effects of Anakinra on LPS- and AC-Induced Neuroinflammation. / Retinasamy, Thaarvena; Lee, Amber Lot Yee; Lee, Hsien Siang et al.
In: ACS Chemical Neuroscience, 30.08.2024.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Repurposing Anakinra for Alzheimer's Disease: The In Vitro and In Vivo Effects of Anakinra on LPS- and AC-Induced Neuroinflammation.

AU - Retinasamy, Thaarvena

AU - Lee, Amber Lot Yee

AU - Lee, Hsien Siang

AU - Lee, Vanessa Lin Lin

AU - Shaikh, Mohd Farooq

AU - Yeong, Keng Yoon

PY - 2024/8/30

Y1 - 2024/8/30

N2 - Alzheimer's disease is a significant global health issue, and studies suggest that neuroinflammation plays a vital role in the advancement of this disease. In this study, anakinra has been shown to display a time- and concentration-dependent antineuroinflammatory effect. In the in vitro studies, it diminished the gene expressions of tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO) synthase 2 stimulated by lipopolysaccharide (LPS). Anakinra also reduced the LPS-induced production of NO and reactive oxygen species. Thus, the hypertrophic state of LPS-activated BV2 microglial cells was reversed by anakinra. Furthermore, acrylamide (ACR)-induced activation of nuclear transcription factor-κB, TNF-α, and interleukin-1β was downregulated, while cAMP response element binding protein and brain-derived neurotrophic factor expression levels were markedly enhanced in ACR-treated zebrafish larvae. It was also observed that anakinra improved the uncoordinated swimming behaviors in ACR-exposed zebrafish larvae. Overall, anakinra demonstrated potential antineuroinflammatory and antioxidative effects.

AB - Alzheimer's disease is a significant global health issue, and studies suggest that neuroinflammation plays a vital role in the advancement of this disease. In this study, anakinra has been shown to display a time- and concentration-dependent antineuroinflammatory effect. In the in vitro studies, it diminished the gene expressions of tumor necrosis factor-alpha (TNF-α) and nitric oxide (NO) synthase 2 stimulated by lipopolysaccharide (LPS). Anakinra also reduced the LPS-induced production of NO and reactive oxygen species. Thus, the hypertrophic state of LPS-activated BV2 microglial cells was reversed by anakinra. Furthermore, acrylamide (ACR)-induced activation of nuclear transcription factor-κB, TNF-α, and interleukin-1β was downregulated, while cAMP response element binding protein and brain-derived neurotrophic factor expression levels were markedly enhanced in ACR-treated zebrafish larvae. It was also observed that anakinra improved the uncoordinated swimming behaviors in ACR-exposed zebrafish larvae. Overall, anakinra demonstrated potential antineuroinflammatory and antioxidative effects.

U2 - 10.1021/acschemneuro.4c00205

DO - 10.1021/acschemneuro.4c00205

M3 - Article

C2 - 39213521

SN - 1948-7193

JO - ACS Chemical Neuroscience

JF - ACS Chemical Neuroscience

ER -

Retinasamy T, Lee ALY, Lee HS, Lee VLL, Shaikh MF, Yeong KY. Repurposing Anakinra for Alzheimer's Disease: The In Vitro and In Vivo Effects of Anakinra on LPS- and AC-Induced Neuroinflammation. ACS Chemical Neuroscience. 2024 Aug 30. doi: 10.1021/acschemneuro.4c00205